| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Drozdowski, Jessica L (13)
|
NITWST |
2:31.27 |
2:32.29 |
1.02 |
| 2 |
Sauer, Emma L (13)
|
NIHAMB |
2:42.46 |
2:40.03 |
-2.43 |
| 3 |
Kubiak, Kelly A (14)
|
NITWST |
2:44.43 |
2:40.51 |
-3.92 |
| 4 |
Kwitek, Grace M (14)
|
NIEAST |
2:44.27 |
2:46.83 |
2.56 |
| 5 |
Frerichs, Greta L (13)
|
NIHHSC |
2:47.67 |
2:47.37 |
-0.30 |
| 6 |
Sainsbury, Meghan A (13)
|
NITWST |
2:52.28 |
2:52.13 |
-0.15 |
| 7 |
Machowski, Stella J (14)
|
NITWST |
2:58.25 |
2:53.19 |
-5.06 |
| 8 |
Mancuso, Gabriella J (13)
|
NIHAMB |
3:18.29 |
3:00.92 |
-17.37 |
| 9 |
Blank, Sabrina E (14)
|
NITWST |
3:19.40 |
3:13.80 |
-5.60 |
|
Barry, Caitlin E (14)
|
NITWST |
3:07.15 |
NS |
- |
|
Musterait, Mallory E (13)
|
NITWST |
2:40.95 |
NS |
- |
|
Regan, Maggie E (13)
|
NITWST |
3:35.33 |
NS |
- |